LOCUS       AAV2_hSyn_ReaCh_mC        6375 bp ds-DNA   circular     26-JUL-2013
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    570..1041
                     /label=hSyn1 promoter
                     /ApEinfo_fwdcolor=#a3ff98
                     /ApEinfo_revcolor=green
     misc_feature    2131..2847
                     /label=Citrine
                     /ApEinfo_fwdcolor=#efff00
                     /ApEinfo_revcolor=green
     misc_feature    2878..3482
                     /label=WPRE
                     /ApEinfo_fwdcolor=#47ff46
                     /ApEinfo_revcolor=green
     misc_feature    1072..1080
                     /label=Kozak seq
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    1065..1070
                     /label=AgeI
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
     misc_feature    4697..5556
                     /label=Amp
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    378..509
                     /label=5'ITR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    3804..3934
                     /label=3' ITR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    4116..4433
                     /label=f1 origin
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
     misc_feature    3521..3733
                     /label=SV40 polyA 
                     /ApEinfo_fwdcolor=#996633
                     /ApEinfo_revcolor=green
     misc_feature    1075..2124
                     /label=ReaChR
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
     misc_feature    1059..1064
                     /label=BamHI
                     /ApEinfo_fwdcolor=#996633
                     /ApEinfo_revcolor=green
     misc_feature    1051..1056
                     /label=NheI
                     /ApEinfo_fwdcolor=#996633
                     /ApEinfo_revcolor=green
     misc_feature    2125..2130
                     /label=XhoI
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    2872..2877
                     /label=HindIII
                     /ApEinfo_fwdcolor=#996633
                     /ApEinfo_revcolor=green
     misc_feature    2851..2856
                     /label=NheI(1)
                     /ApEinfo_label=NheI
                     /ApEinfo_fwdcolor=#996633
                     /ApEinfo_revcolor=green
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccagattt
      361 aattaaggcc ttaattaggc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc
      421 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg
      481 agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt
      541 atctacgtag ccatgctcta ggaAGATCTc tgcagagggc cctgcgtatg agtgcaagtg
      601 ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccacccac
      661 tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag agggggaggg
      721 gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt
      781 cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac
      841 tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg
      901 gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc gaccatctgc
      961 gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt
     1021 cgtgcctgag agcgcagtcg aattcaagct gctagcaagg atccACCGGT GACCATGGTG
     1081 AGCAGAAGAC CCTGGCTGCT GGCCCTGGCC CTGGCCGTGG CCCTGGCCGC CGGCAGCGCC
     1141 GGCGCCAGCA CCGGCAGCGA CGCCACCGTG CCCGTGGCCA CCCAGGACGG CCCCGACTAC
     1201 GTGTTCCACA GAGCCCACGA GAGAATGCTG TTCCAGACCA GCTACACCCT GGAGAACAAC
     1261 GGCAGCGTGA TCTGCATCCC CAACAACGGC CAGTGCTTCT GCCTGGCCTG GCTGAAGAGT
     1321 AACGGCACCA ACGCCGAGAA GCTGGCCGCC AACATCCTGC AGTGGGTGGT GTTTGCGCTG
     1381 AGCGTGGCGT GCCTGGGCTG GTATGCGTAT CAGGCGTGGC GCGCGACCTG CGGCTGGGAA
     1441 GAAGTGTATG TGGCGCTGAT TGAAATGATG AAAAGCATTA TTGAAGCGTT TCATGAATTT
     1501 GATAGCCCGG CGACCCTGTG GCTGAGCAGC GGCAACGGCG TGGTGTGGAT GCGCTATGGC
     1561 GAATGGCTGC TGACCTGCCC CGTGattCTG ATTcatCTGA GCAACCTGAC CGGCCTGAAA
     1621 GATGATTATA GCAAACGCAC CATGGGCCTG CTGGTGAGCG ACGTGGGCTG CATTGTGTGG
     1681 GGCGCGACCA GCGCGATGTG CACCGGCTGG ACCAAAATTC TGTTTTTTCT GATTAGCCTG
     1741 AGCTATGGCA TGTATACCTA TTTTCATGCG GCCAAAGTGT ATATTGAAGC GTTTCATACC
     1801 GTGCCGAAAG GCCTGTGCAG ACAGCTGGTG AGAGCCATGG CCTGGCTGTT CTTCGTGAGC
     1861 TGGGGCATGT TCCCCGTGCT GTTCCTGCTG GGCCCCGAGG GCTTCGGCCA TATTAGCCCG
     1921 TATGGCAGCG CGATTGGCCA TAGCATTCTG GATCTGATTG CGAAGAACAT GTGGGGCGTG
     1981 CTGGGCAACT ATCTGCGCGT GAAAATTCAT GAACATATTC TGCTGTATGG CGATATTCGC
     2041 AAAAAACAGA AAATTACCAT TGCGGGCCAG GAAATGGAAG TGGAAACCCT GGTGGCGGAA
     2101 GAAGAAGATA AGTACGAGAG CAGCCTCGAG atggtgagca agggcgagga gctgttcacc
     2161 ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg
     2221 tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc
     2281 accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccttcggcta cggcctgatg
     2341 tgcttcgccc gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc
     2401 gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc
     2461 gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac
     2521 ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac
     2581 gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac
     2641 aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc
     2701 gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc cctgagcaaa
     2761 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     2821 actctcggca tggacgagct gtacaagTAG GCTAGCcccg aattcgatat caagcttatc
     2881 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     2941 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3001 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3061 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3121 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3181 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3241 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     3301 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3361 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3421 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     3481 cgtcgacctc gacccgggcg gccgcttcga gcagacatga taagatacat tgatgagttt
     3541 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     3601 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     3661 cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc
     3721 tacaaatgtg gtaaaatcga taaggatctt cctagagcat ggctacgtag ataagtagca
     3781 tggcgggtta atcattaact acaaggaacc cctagtgatg gagttggcca ctccctctct
     3841 gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc
     3901 ccgggcggcc tcagtgagcg agcgagcgcg cagccttaat taacctaatt cactggccgt
     3961 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
     4021 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
     4081 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
     4141 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     4201 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     4261 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     4321 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
     4381 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     4441 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     4501 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     4561 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
     4621 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
     4681 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
     4741 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
     4801 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
     4861 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
     4921 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat
     4981 tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg
     5041 acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta
     5101 cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat
     5161 catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag
     5221 cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa
     5281 ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca
     5341 ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc
     5401 ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt
     5461 atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc
     5521 gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat
     5581 atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt
     5641 tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac
     5701 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
     5761 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
     5821 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
     5881 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
     5941 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
     6001 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     6061 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     6121 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     6181 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     6241 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     6301 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     6361 ccttttgctc acatg    
//
