LOCUS       AAV2_hSyn_oChIEF_t        7118 bp ds-DNA   circular     05-FEB-2014
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    570..1041
                     /label=hSyn
                     /ApEinfo_fwdcolor=#a3ff98
                     /ApEinfo_revcolor=green
     misc_feature    join(3610..4073,4085..4225)
                     /label=WPRE
                     /ApEinfo_fwdcolor=#47ff46
                     /ApEinfo_revcolor=green
     misc_feature    2146..2151
                     /label=WPRE(1)
                     /ApEinfo_label=WPRE
                     /ApEinfo_fwdcolor=#47ff46
                     /ApEinfo_revcolor=green
     misc_feature    1059..1064
                     /label=BamHI
                     /ApEinfo_fwdcolor=#ff7ec1
                     /ApEinfo_revcolor=green
     misc_feature    4074..4084
                     /label=f1 origin
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
     misc_feature    2152..2829
                     /label=Tomato copy 1
                     /ApEinfo_fwdcolor=#ff0600
                     /ApEinfo_revcolor=green
     misc_feature    5440..6299
                     /label=Amp
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    2899..3555
                     /label=Tomato copy 2
                     /ApEinfo_fwdcolor=#ff5c1c
                     /ApEinfo_revcolor=green
     misc_feature    378..509
                     /label=5'ITR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    4547..4677
                     /label=3' ITR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    3592..3597
                     /label=EcoRI
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    4859..5176
                     /label=f1 origin(1)
                     /ApEinfo_label=f1 origin
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
     misc_feature    2152..3582
                     /label=tdTomato
                     /ApEinfo_fwdcolor=#fffb30
                     /ApEinfo_revcolor=green
     misc_feature    4264..4476
                     /label=SV40 polyA 
                     /ApEinfo_fwdcolor=#996633
                     /ApEinfo_revcolor=green
     misc_feature    1066..1074
                     /label=Kozak sequence
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    1069..2145
                     /label=oChIEF
                     /ApEinfo_fwdcolor=#ff9da9
                     /ApEinfo_revcolor=green
     misc_feature    3583..3589
                     /label=NheI
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    3604..3609
                     /label=HindIII
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccagattt
      361 aattaaggcc ttaattaggc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc
      421 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg
      481 agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt
      541 atctacgtag ccatgctcta ggaAGATCTc tgcagagggc cctgcgtatg agtgcaagtg
      601 ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccacccac
      661 tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag agggggaggg
      721 gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt
      781 cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac
      841 tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg
      901 gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc gaccatctgc
      961 gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt
     1021 cgtgcctgag agcgcagtcg aattcaagct gctagcaagg atccGACCAT GGTGAGCAGA
     1081 AGACCCTGGC TGCTGGCCCT GGCCCTGGCC GTGGCCCTGG CCGCCGGCAG CGCCGGCGCC
     1141 AGCACCGGCA GCGACGCCAC CGTGCCCGTG GCCACCCAGG ACGGCCCCGA CTACGTGTTC
     1201 CACAGAGCCC ACGAGAGAAT GCTGTTCCAG ACCAGCTACA CCCTGGAGAA CAACGGCAGC
     1261 GTGATCTGCA TCCCCAACAA CGGCCAGTGC TTCTGCCTGG CCTGGCTGAA GAGTAACGGC
     1321 ACCAACGCCG AGAAGCTGGC CGCCAACATC CTGCAGTGGA TCACCTTCGC CCTGAGCGCC
     1381 CTGTGCCTGA TGTTCTACGG CTACCAGACC TGGAAGAGTA CCTGCGGCTG GGAGGAGATC
     1441 TACGTGGCCA CCATCGAGAT GATCAAGTTC ATCATAGAGT ACTTCCACGA GTTCGACGAG
     1501 CCCGCCGTGA TCTACAGCAG CAACGGCAAC AAGACCGTGT GGCTGAGATA CGCCGAGTGG
     1561 CTGCTGACCT GCCCCGTGGT CCTGATCCAC CTGAGCAACC TGACCGGCCT GGCCAACGAC
     1621 TACAACAAGA GAACCATGGG CCTGCTGGTG AGCGACATCG GCACCATCGT GTGGGGCACC
     1681 ACCGCCGCCC TGAGCAAGGG CTACGTGAGA GTGATCTTCT TCCTGATGGG CCTGTGCTAC
     1741 GGCATCTACA CCTTCTTCAA CGCCGCCAAG GTGTACATCG AGGCCTACCA CACCGTGCCC
     1801 AAGGGCAGAT GCAGACAGGT GGTGACCGGC ATGGCCTGGC TGTTCTTCGT GAGCTGGGGC
     1861 ATGTTCCCCA TCCTGTTCAT CCTGGGCCCC GAGGGCTTCG GCGTGCTGAG CGTGTACGGC
     1921 AGCACCGTGG GCCACACCAT CATCGACCTG ATGAGCAAGA ACTGCTGGGG CCTGCTGGGC
     1981 CACTACCTGA GAGTGCTGAT CCACGAGCAC ATCCTGATCC ACGGCGACAT CAGAAAGACC
     2041 ACCAAGCTGA ACATCGGCGG CACCGAGATC GAGGTGGAGA CCCTGGTGGA GGACGAGGCC
     2101 GAGGCCGGCG CCGTGAACAA GGGCACCGGC AAGTACGAGA GCAGCatcga tatggtgagc
     2161 aagggcgagg aggtcatcaa agagttcatg cgcttcaagg tgcgcatgga gggctccatg
     2221 aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag
     2281 accgccaagc tgaaggtgac caagggcggc cccctgccct tcgcctggga catcctgtcc
     2341 ccccagttca tgtacggctc caaggcgtac gtgaagcacc ccgccgacat ccccgattac
     2401 aagaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc
     2461 ggtctggtga ccgtgaccca ggactcctcc ctgcaggacg gcacgctgat ctacaaggtg
     2521 aagatgcgcg gcaccaactt cccccccgac ggccccgtaa tgcagaagaa gaccatgggc
     2581 tgggaggcct ccaccgagcg cctgtacccc cgcgacggcg tgctgaaggg cgagatccac
     2641 caggccctga agctgaagga cggcggccac tacctggtgg agttcaagac catctacatg
     2701 gccaagaagc ccgtgcaact gcccggctac tactacgtgg acaccaagct ggacatcacc
     2761 tcccacaacg aggactacac catcgtggaa cagtacgagc gctccgaggg ccgccaccac
     2821 ctgttcctgg ggcatggcac cggcagcacc ggcagcggca gctccggcac cgcctcctcc
     2881 gaggacaaca acatggccgt catcaaagag ttcatgcgct tcaaggtgcg catggagggc
     2941 tccatgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
     3001 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
     3061 ctgtcccccc agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc
     3121 gattacaaga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
     3181 gacggcggtc tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac
     3241 aaggtgaaga tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc
     3301 atgggctggg aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag
     3361 atccaccagg ccctgaagct gaaggacggc ggccactacc tggtggagtt caagaccatc
     3421 tacatggcca agaagcccgt gcaactgccc ggctactact acgtggacac caagctggac
     3481 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgctc cgagggccgc
     3541 caccacctgt tcctgtacgg catggacgag ctgtacaagt agGCTAGCcc cgaattcgat
     3601 atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     3661 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     3721 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     3781 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     3841 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     3901 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     3961 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     4021 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttAACTGGA
     4081 ACAActgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc
     4141 cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt
     4201 gggccgcctc cccgcatcga taccgtcgac ctcgacccgg gcggccgctt cgagcagaca
     4261 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     4321 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     4381 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag atgtgggagg
     4441 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat cttcctagag
     4501 catggctacg tagataagta gcatggcggg ttaatcatta actacaagga acccctagtg
     4561 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag
     4621 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagcctt
     4681 aattaaccta attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
     4741 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
     4801 gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc
     4861 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     4921 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     4981 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5041 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5101 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5161 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     5221 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     5281 tttaacaaaa tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa
     5341 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     5401 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     5461 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     5521 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     5581 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5641 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5701 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5761 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5821 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5881 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5941 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     6001 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     6061 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     6121 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     6181 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     6241 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     6301 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     6361 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     6421 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6481 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6541 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6601 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6661 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6721 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6781 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6841 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6901 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6961 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     7021 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     7081 tacggttcct ggccttttgc tggccttttg ctcacatg  
//
