LOCUS       AAV2_hSyn_mSOG_VAM        6027 bp ds-DNA   circular     26-JUL-2013
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    570..1041
                     /label=hSyn1 promoter
                     /ApEinfo_fwdcolor=#a3ff98
                     /ApEinfo_revcolor=green
     misc_feature    1424..1792
                     /label=Vamp2
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    1067..1384
                     /label=miniSOG
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
     misc_feature    1769..1792
                     /label=linker
                     /ApEinfo_fwdcolor=#ff8573
                     /ApEinfo_revcolor=green
     misc_feature    1799..2515
                     /label=Citrine
                     /ApEinfo_fwdcolor=#efff00
                     /ApEinfo_revcolor=green
     misc_feature    2530..3134
                     /label=WPRE
                     /ApEinfo_fwdcolor=#47ff46
                     /ApEinfo_revcolor=green
     misc_feature    4349..5208
                     /label=Amp
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    3173..3385
                     /label=SV40 polyA 
                     /ApEinfo_fwdcolor=#996633
                     /ApEinfo_revcolor=green
     misc_feature    378..509
                     /label=5'ITR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    3456..3586
                     /label=3' ITR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    3768..4085
                     /label=f1 origin
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
     misc_feature    1418..1423
                     /label=AgeI
                     /ApEinfo_fwdcolor=#996633
                     /ApEinfo_revcolor=green
     misc_feature    1059..1064
                     /label=BamHI
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    1385..1417
                     /label=C-terminal linker
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
     misc_feature    1793..1798
                     /label=ClaI
                     /ApEinfo_fwdcolor=#996633
                     /ApEinfo_revcolor=green
     misc_feature    2524..2529
                     /label=HindIII
                     /ApEinfo_fwdcolor=#996633
                     /ApEinfo_revcolor=green
     misc_feature    1051..1056
                     /label=NheI
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccagattt
      361 aattaaggcc ttaattaggc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc
      421 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg
      481 agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt
      541 atctacgtag ccatgctcta ggaAGATCTc tgcagagggc cctgcgtatg agtgcaagtg
      601 ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccacccac
      661 tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag agggggaggg
      721 gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt
      781 cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac
      841 tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg
      901 gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc gaccatctgc
      961 gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt
     1021 cgtgcctgag agcgcagtcg aattcaagct gctagcaaGG ATCCtcATgG AGAAAAGTTT
     1081 CGTGATAAcT GATCCACGGC TGCCAGACAA TCCCATCATC TTCGCATCCG ATggCTTCCT
     1141 GGAGCTGACC GAGTATTCCA GAGAGGAGAT CCTGGGCCGC AATGgCCGCT TTCTGCAGGG
     1201 ACCAGAGACA GACCAGGCCA CAGTGCAGAA GATTCGCGAT GCCATTAGAG ATCAGCGCGA
     1261 GATTACCGTG CAGCTGATAA ACTACACAAA AAGCGGGAAG AAATTCTGGA ACCTCcTgCA
     1321 CCTCCAGCCC ATGAGGGACC AGAAGGGTGA GCTCCAGTAT TTCATCGGAG TGCAGCTGGA
     1381 TGGATCAGGA GGAGGCTCAG GAGGAGGAGG CGCGCCTACC GGTATGTCGG CTACCGCTGC
     1441 CACCGTCCCG CCTGCCGCCC CGGCCGGCGA GGGTGGCCCC CCTGCACCTC CTCCAAACCT
     1501 TACTAGTAAC AGGAGACTGC AGCAGACCCA GGCCCAGGTG GATGAGGTGG TGGACATCAT
     1561 GAGGGTGAAT GTGGACAAGG TCCTGGAGCG GGACCAGAAG TTGTCGGAGC TGGATGACCG
     1621 TGCAGATGCC CTCCAGGCAG GGGCCTCCCA GTTTGAAACA AGTGCAGCCA AGCTCAAGCG
     1681 CAAATACTGG TGGAAAAACC TCAAGATGAT GATCATCTTG GGAGTGATCT GCGCCATCAT
     1741 CCTCATCATC ATCATCGTTT ACTTCAGCAC TAGCGGCGGA AGCGGCGGGG GAATCGATat
     1801 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     1861 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     1921 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     1981 cgtgaccacc ttcggctacg gcctgatgtg cttcgcccgc taccccgacc acatgaagca
     2041 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     2101 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     2161 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     2221 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     2281 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     2341 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     2401 cctgagctac cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     2461 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagTAGAA
     2521 TTCAAGCTTa tcgataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     2581 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     2641 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     2701 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     2761 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     2821 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     2881 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     2941 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     3001 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     3061 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     3121 ccgcatcgat accgtcgacc tcgacccggg cggccgcttc gagcagacat gataagatac
     3181 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
     3241 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac
     3301 aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt tttttaaagc
     3361 aagtaaaacc tctacaaatg tggtaaaatc gataaggatc ttcctagagc atggctacgt
     3421 agataagtag catggcgggt taatcattaa ctacaaggaa cccctagtga tggagttggc
     3481 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     3541 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgcagcctta attaacctaa
     3601 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     3661 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     3721 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     3781 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     3841 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     3901 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     3961 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     4021 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     4081 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     4141 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     4201 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     4261 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     4321 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     4381 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     4441 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     4501 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     4561 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     4621 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     4681 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     4741 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     4801 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     4861 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     4921 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     4981 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     5041 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     5101 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     5161 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     5221 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     5281 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     5341 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     5401 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     5461 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     5521 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     5581 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     5641 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     5701 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     5761 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     5821 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     5881 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     5941 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     6001 gccttttgct ggccttttgc tcacatg   
//
