LOCUS       AAV2_hSyn_mSOG_VAM        6071 bp ds-DNA   circular     26-JUL-2013
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    570..1041
                     /label=hSyn1 promoter
                     /ApEinfo_fwdcolor=#a3ff98
                     /ApEinfo_revcolor=green
     misc_feature    1068..1385
                     /label=miniSOG
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
     misc_feature    1059..1064
                     /label=BamHI
                     /ApEinfo_fwdcolor=#f5ff7b
                     /ApEinfo_revcolor=green
     misc_feature    2568..2573
                     /label=HindIII
                     /ApEinfo_fwdcolor=#fffba2
                     /ApEinfo_revcolor=green
     misc_feature    2574..3178
                     /label=WPRE
                     /ApEinfo_fwdcolor=#47ff46
                     /ApEinfo_revcolor=green
     misc_feature    1785..1838
                     /vntifkey="21"
                     /label=T2A
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
     misc_feature    4393..5252
                     /label=Amp
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    1764..1784
                     /vntifkey="21"
                     /label=GSG\linker
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
     misc_feature    3217..3429
                     /label=SV40 polyA 
                     /ApEinfo_fwdcolor=#996633
                     /ApEinfo_revcolor=green
     misc_feature    378..509
                     /label=5'ITR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     CDS             1845..2555
                     /vntifkey="4"
                     /label=mCherry
                     /ApEinfo_fwdcolor=pink
                     /ApEinfo_revcolor=pink
     misc_feature    3500..3630
                     /label=3' ITR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    3812..4129
                     /label=f1 origin
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
     misc_feature    1386..1409
                     /label=C-term linker
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=green
     misc_feature    1410..1757
                     /label=Vamp2
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    1758..1763
                     /label=NheI
                     /ApEinfo_fwdcolor=#996633
                     /ApEinfo_revcolor=green
     misc_feature    1051..1056
                     /label=NheI(1)
                     /ApEinfo_label=NheI
                     /ApEinfo_fwdcolor=#996633
                     /ApEinfo_revcolor=green
     misc_feature    1839..1844
                     /label=BsiWI
                     /ApEinfo_fwdcolor=#996633
                     /ApEinfo_revcolor=green
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccagattt
      361 aattaaggcc ttaattaggc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc
      421 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg
      481 agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt
      541 atctacgtag ccatgctcta ggaAGATCTc tgcagagggc cctgcgtatg agtgcaagtg
      601 ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccacccac
      661 tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag agggggaggg
      721 gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt
      781 cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac
      841 tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg
      901 gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc gaccatctgc
      961 gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt
     1021 cgtgcctgag agcgcagtcg aattcaagct gctagcaagg atccggtATg GAGAAAAGTT
     1081 TCGTGATAAc TGATCCACGG CTGCCAGACA ATCCCATCAT CTTCGCATCC GATggCTTCC
     1141 TGGAGCTGAC CGAGTATTCC AGAGAGGAGA TCCTGGGCCG CAATGgCCGC TTTCTGCAGG
     1201 GACCAGAGAC AGACCAGGCC ACAGTGCAGA AGATTCGCGA TGCCATTAGA GATCAGCGCG
     1261 AGATTACCGT GCAGCTGATA AACTACACAA AAAGCGGGAA GAAATTCTGG AACCTCcTgC
     1321 ACCTCCAGCC CATGAGGGAC CAGAAGGGTG AGCTCCAGTA TTTCATCGGA GTGCAGCTGG
     1381 ATGGATCAGG AGGAGGCTCA GGAGGAGGAA TGTCGGCTAC CGCTGCCACC GTCCCGCCTG
     1441 CCGCCCCGGC CGGCGAGGGT GGCCCCCCTG CACCTCCTCC AAACCTTACT AGTAACAGGA
     1501 GACTGCAGCA GACCCAGGCC CAGGTGGATG AGGTGGTGGA CATCATGAGG GTGAATGTGG
     1561 ACAAGGTCCT GGAGCGGGAC CAGAAGTTGT CGGAGCTGGA TGACCGTGCA GATGCCCTCC
     1621 AGGCAGGGGC CTCCCAGTTT GAAACAAGTG CAGCCAAGCT CAAGCGCAAA TACTGGTGGA
     1681 AAAACCTCAA GATGATGATC ATCTTGGGAG TGATCTGCGC CATCATCCTC ATCATCATCA
     1741 TCGTTTACTT CAGCACTgct agcggaggtg gatctggtgg cggtgagggc agaggaagtc
     1801 ttctaacatg cggtgacgtg gaggagaatc ccggccctcg tacgatggtg agcaagggcg
     1861 aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct
     1921 ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca
     1981 cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc
     2041 tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg
     2101 actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg
     2161 acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca
     2221 aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca
     2281 tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga
     2341 tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct
     2401 acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca
     2461 tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc
     2521 actccaccgg cggcatggac gagctgtaca agtaagaatt cgatatcAAG CTTatcgata
     2581 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     2641 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     2701 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     2761 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     2821 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     2881 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     2941 tgggcactga caattccgtg gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg
     3001 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3061 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3121 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgataccgtc
     3181 gacctcgacc cgggcggccg cttcgagcag acatgataag atacattgat gagtttggac
     3241 aaaccacaac tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg
     3301 ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt
     3361 ttatgtttca ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca
     3421 aatgtggtaa aatcgataag gatcttccta gagcatggct acgtagataa gtagcatggc
     3481 gggttaatca ttaactacaa ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     3541 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     3601 gcggcctcag tgagcgagcg agcgcgcagc cttaattaac ctaattcact ggccgtcgtt
     3661 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     3721 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     3781 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     3841 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     3901 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     3961 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4021 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4081 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     4141 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     4201 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     4261 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4321 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4381 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4441 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4501 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4561 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4621 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4681 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4741 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4801 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4861 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4921 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4981 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5041 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5101 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5161 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5221 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5281 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5341 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5401 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5461 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5521 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5581 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5641 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5701 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5761 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5821 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5881 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5941 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6001 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6061 ttgctcacat g    
//
