LOCUS       AAV2_hSyn_SYP1_mSO        6668 bp ds-DNA   circular     26-JUL-2013
DEFINITION  .
ACCESSION   
VERSION     
SOURCE      .
  ORGANISM  .
COMMENT     
COMMENT     ApEinfo:methylated:1
FEATURES             Location/Qualifiers
     misc_feature    570..1041
                     /label=hSyn1 promoter
                     /ApEinfo_fwdcolor=#a3ff98
                     /ApEinfo_revcolor=green
     misc_feature    1059..1064
                     /label=BamHI
                     /ApEinfo_fwdcolor=#f5ff7b
                     /ApEinfo_revcolor=green
     misc_feature    3165..3170
                     /label=HindIII
                     /ApEinfo_fwdcolor=#fffba2
                     /ApEinfo_revcolor=green
     misc_feature    3171..3775
                     /label=WPRE
                     /ApEinfo_fwdcolor=#47ff46
                     /ApEinfo_revcolor=green
     misc_feature    2361..2381
                     /vntifkey="21"
                     /label=GSG\linker
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=green
     misc_feature    2382..2435
                     /vntifkey="21"
                     /label=T2A
                     /ApEinfo_fwdcolor=#0000ff
                     /ApEinfo_revcolor=green
     CDS             2442..3152
                     /vntifkey="4"
                     /label=mCherry
                     /ApEinfo_fwdcolor=#ff0000
                     /ApEinfo_revcolor=pink
     misc_feature    4990..5849
                     /label=Amp
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     exon            1083..2006
                     /vntifkey="61"
                     /label=Synaptophysin
                     /ApEinfo_fwdcolor=#00ff00
                     /ApEinfo_revcolor=pink
     misc_feature    378..509
                     /label=5'ITR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     exon            2037..2354
                     /vntifkey="61"
                     /label=mSOG
                     /ApEinfo_fwdcolor=#ffff00
                     /ApEinfo_revcolor=pink
     misc_feature    4097..4227
                     /label=3' ITR
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    3814..4026
                     /label=SV40 polyA 
                     /ApEinfo_fwdcolor=#996633
                     /ApEinfo_revcolor=green
     misc_feature    4409..4726
                     /label=f1 origin
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
     misc_feature    2007..2036
                     /label=Linker
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    2355..2381
                     /label=linker
                     /ApEinfo_fwdcolor=cyan
                     /ApEinfo_revcolor=green
     misc_feature    2436..2441
                     /label=BsiWI
                     /ApEinfo_fwdcolor=#800080
                     /ApEinfo_revcolor=green
     misc_feature    2355..2360
                     /label=NheI
                     /ApEinfo_fwdcolor=#ff8000
                     /ApEinfo_revcolor=green
     misc_feature    2007..2012
                     /label=AgeI
                     /ApEinfo_fwdcolor=#996633
                     /ApEinfo_revcolor=green
     misc_feature    1051..1056
                     /label=NheI(1)
                     /ApEinfo_label=NheI
                     /ApEinfo_fwdcolor=#996633
                     /ApEinfo_revcolor=green
ORIGIN
        1 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
       61 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
      121 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
      181 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      241 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      301 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccagattt
      361 aattaaggcc ttaattaggc tgcgcgctcg ctcgctcact gaggccgccc gggcaaagcc
      421 cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc gcagagaggg
      481 agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc catgctactt
      541 atctacgtag ccatgctcta ggaAGATCTc tgcagagggc cctgcgtatg agtgcaagtg
      601 ggttttagga ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccacccac
      661 tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag agggggaggg
      721 gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt
      781 cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac
      841 tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg
      901 gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc gaccatctgc
      961 gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt
     1021 cgtgcctgag agcgcagtcg aattcaagct gctagcaagg atccgtgacc ggcggctcta
     1081 gaatggacgt ggtgaatcag ctggtggctg ggggtcagtt ccgggtggtc aaggagcccc
     1141 ttggcttcgt gaaggtgctg cagtgggtct ttgccatctt cgcctttgct acgtgtggca
     1201 gctacaccgg ggagcttcgg ctgagcgtgg agtgtgccaa caagacggag agtgccctca
     1261 acatcgaagt tgaattcgag taccccttca ggctgcacca agtgtacttt gatgcaccct
     1321 cctgcgtcaa agggggcact accaagatct tcctggttgg ggactactcc tcgtcggctg
     1381 aattctttgt caccgtggct gtgtttgcct tcctctactc catgggggcc ctggccacct
     1441 acatcttcct gcagaacaag taccgagaga acaacaaagg gcctatgatg gactttctgg
     1501 ctacagccgt gttcgctttc atgtggctag ttagttcatc agcctgggcc aaaggcctgt
     1561 ccgatgtgaa gatggccacg gacccagaga acattatcaa ggagatgccc atgtgccgcc
     1621 agacagggaa cacatgcaag gaactgaggg accctgtgac ttcaggactc aacacctcag
     1681 tggtgtttgg cttcctgaac ctggtgctct gggttggcaa cttatggttc gtgttcaagg
     1741 agacaggctg ggcagcccca ttcatgcgcg cacctccagg cgccccggaa aagcaaccag
     1801 cacctggcga tgcctacggc gatgcgggct acgggcaggg ccccggaggc tatgggcccc
     1861 aggactccta cgggcctcag ggtggttatc aacccgatta cgggcagcca gccagcggtg
     1921 gcggtggcta cgggcctcag ggcgactatg ggcagcaagg ctatggccaa cagggtgcgc
     1981 ccacctcctt ctccaatcag atgaaaaccg gtggtggcgg cagtggtggc ggcagcatgg
     2041 agaaaagttt cgtgataact gatccacggc tgccagacaa tcccatcatc ttcgcatccg
     2101 atggcttcct ggagctgacc gagtattcca gagaggagat cctgggccgc aatggccgct
     2161 ttctgcaggg accagagaca gaccaggcca cagtgcagaa gattcgcgat gccattagag
     2221 atcagcgcga gattaccgtg cagctgataa actacacaaa aagcgggaag aaattctgga
     2281 acctcctgca cctccagccc atgagggacc agaagggtga gctccagtat ttcatcggag
     2341 tgcagctgga tggagctagc ggaggtggat ctggtggcgg tgagggcaga ggaagtcttc
     2401 taacatgcgg tgacgtggag gagaatcccg gccctcgtac gatggtgagc aagggcgagg
     2461 aggataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg
     2521 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc
     2581 agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
     2641 cccctcagtt catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
     2701 acttgaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg
     2761 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
     2821 tgaagctgcg cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg
     2881 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca
     2941 agcagaggct gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca
     3001 aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca
     3061 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
     3121 ccaccggcgg catggacgag ctgtacaagt aagaattcga tatcAAGCTT atcgataatc
     3181 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     3241 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     3301 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     3361 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     3421 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     3481 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     3541 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     3601 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     3661 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     3721 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac
     3781 ctcgacccgg gcggccgctt cgagcagaca tgataagata cattgatgag tttggacaaa
     3841 ccacaactag aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
     3901 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
     3961 tgtttcaggt tcagggggag atgtgggagg ttttttaaag caagtaaaac ctctacaaat
     4021 gtggtaaaat cgataaggat cttcctagag catggctacg tagataagta gcatggcggg
     4081 ttaatcatta actacaagga acccctagtg atggagttgg ccactccctc tctgcgcgct
     4141 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
     4201 gcctcagtga gcgagcgagc gcgcagcctt aattaaccta attcactggc cgtcgtttta
     4261 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     4321 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4381 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4441 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4501 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4561 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4621 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4681 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4741 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4801 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     4861 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4921 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     4981 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5041 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5101 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5161 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5221 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5281 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5341 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5401 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5461 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5521 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5581 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5641 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5701 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5761 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5821 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5881 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     5941 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6001 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6061 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6121 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6181 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6241 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6301 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6361 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6421 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6481 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6541 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6601 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6661 ctcacatg     
//
